Stains, Chemical Solution
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Stains, Chemical Solution
BECTON, DICKINSON AND COMPANY | ||||
CATALOG # | QTY | PRICE | DESCRIPTION | |
212517 | 0 | - | ||
212528 | 0 | - |
Fertipro NV | ||||
CATALOG # | QTY | PRICE | DESCRIPTION | |
LEUCO | 0 | - | ||
LEUCO_1 | 0 | - | ||
LEUCO_2 | 0 | - | ||
LEUCO_PLUS | 0 | - | ||
LEUCOP1 | 0 | - | ||
LEUCOP2 | 0 | - | ||
LEUCOP3 | 0 | - | ||
LEUCOP4 | 0 | - | ||
SPS050 | 0 | - | ||
SPS050A | 0 | - | ||
SPS050B | 0 | - | ||
SPS050C | 0 | - | ||
SPS050F | 0 | - | ||
SPS250A | 0 | - | ||
SPS250B | 0 | - | ||
SPS250C | 0 | - | ||
SPS250F | 0 | - | ||
VITAL | 0 | - | ||
VITAL_1 | 0 | - | ||
VITAL_2 | 0 | - |
FISHER SCIENTIFIC COMPANY L.L.C. | ||||
CATALOG # | QTY | PRICE | DESCRIPTION | |
22220107 | 0 | - | ||
22220108 | 0 | - |
HORIBA ABX SAS | ||||
CATALOG # | QTY | PRICE | DESCRIPTION | |
1210904005 | 0 | - | ||
1210904011 | 0 | - |
RICHARD-ALLAN SCIENTIFIC COMPANY | ||||
CATALOG # | QTY | PRICE | DESCRIPTION | |
6769001 | 0 | - | ||
6769002 | 0 | - | ||
6769008 | 0 | - | ||
6769009 | 0 | - | ||
7301L | 0 | - | ||
7402L | 0 | - | ||
88000 | 0 | - | ||
88034 | 0 | - | ||
88035 | 0 | - | ||
88036 | 0 | - | ||
88039 | 0 | - | ||
88041 | 0 | - | ||
88042 | 0 | - | ||
88045 | 0 | - | ||
88047 | 0 | - | ||
88048 | 0 | - | ||
88051 | 0 | - | ||
88052 | 0 | - | ||
88054 | 0 | - | ||
88104 | 0 | - | ||
88116 | 0 | - | ||
TA-006-FM | 0 | - | ||
TA-030-FM | 0 | - | ||
TA-125-AM | 0 | - |
61 Results
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LeucoScreen Reagent 2
Fertipro NV
LeucoScreen is a semi - quantitative histochemical kit for the determination of peroxide-positive white blood cells in human semen.LeucoScreen kit contains two reagents: Reagent 1 - 20ml of LeucoScreen stain (contains: benzidine, cyanosine and methanol) and Reagent 2 - 1ml of 3% Hydrogen peroxide.Almost every semen sample contains cells other than spermatozoa. These may be polygonal cells from the genital tract, but mostly they are nucleated round cells. Round cells are either spermatogenetic precursor cells (spermatids, spermatocytes, sometimes spermatogonia) or white blood cells. Amongst the latter, lymphocytes are rather uncommon, and the majority are polymorphonuclear granulocytes, characterized by granules containing the enzyme peroxidase.LeucoScreen™ differentiates round cells on the basis of their peroxidase content.LeucoScreen™ has a 12 month shelf life from date of produce.LeucoScreen™ has been CE marked according to the specifications set forth in the European Medical Device Directive 98/79/EC. For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen Reagent 1
Fertipro NV
LeucoScreen is a semi - quantitative histochemical kit for the determination of peroxide-positive white blood cells in human semen.LeucoScreen kit contains two reagents: Reagent 1 - 20ml of LeucoScreen stain (contains: benzidine, cyanosine and methanol) and Reagent 2 - 1ml of 3% Hydrogen peroxide.Almost every semen sample contains cells other than spermatozoa. These may be polygonal cells from the genital tract, but mostly they are nucleated round cells. Round cells are either spermatogenetic precursor cells (spermatids, spermatocytes, sometimes spermatogonia) or white blood cells. Amongst the latter, lymphocytes are rather uncommon, and the majority are polymorphonuclear granulocytes, characterized by granules containing the enzyme peroxidase.LeucoScreen™ differentiates round cells on the basis of their peroxidase content.LeucoScreen™ has a 12 month shelf life from date of produce.LeucoScreen™ has been CE marked according to the specifications set forth in the European Medical Device Directive 98/79/EC. For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen
Fertipro NV
LeucoScreen is a semi - quantitative histochemical kit for the determination of peroxide-positive white blood cells in human semen.LeucoScreen kit contains two reagents: Reagent 1 - 20ml of LeucoScreen stain (contains: benzidine, cyanosine and methanol) and Reagent 2 - 1ml of 3% Hydrogen peroxide.Almost every semen sample contains cells other than spermatozoa. These may be polygonal cells from the genital tract, but mostly they are nucleated round cells. Round cells are either spermatogenetic precursor cells (spermatids, spermatocytes, sometimes spermatogonia) or white blood cells. Amongst the latter, lymphocytes are rather uncommon, and the majority are polymorphonuclear granulocytes, characterized by granules containing the enzyme peroxidase.LeucoScreen™ differentiates round cells on the basis of their peroxidase content.LeucoScreen™ has a 12 month shelf life from date of produce.LeucoScreen™ has been CE marked according to the specifications set forth in the European Medical Device Directive 98/79/EC. For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen Plus Reagent 4
Fertipro NV
LeucoScreen Plus™ is an improved histochemical staining kit for the determination of peroxidase-positive white blood cells in semen. The kit provides useful information in semen samples where the concentration of round cells exceeds 1 million/ml. LeucoScreen Plus has an equal capability to distinguish between peroxidase-positive and -negative round cells as the LeucoScreen kit (same assay performance), but has the advantage that the substrate is not carcinogenic. The color of the substrate in peroxidase-positive white blood cells is bluish-grey to black instead of brown (LeucoScreen). For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen Plus Reagent 3
Fertipro NV
LeucoScreen Plus™ is an improved histochemical staining kit for the determination of peroxidase-positive white blood cells in semen. The kit provides useful information in semen samples where the concentration of round cells exceeds 1 million/ml. LeucoScreen Plus has an equal capability to distinguish between peroxidase-positive and -negative round cells as the LeucoScreen kit (same assay performance), but has the advantage that the substrate is not carcinogenic. The color of the substrate in peroxidase-positive white blood cells is bluish-grey to black instead of brown (LeucoScreen). For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen Plus Reagent 2
Fertipro NV
LeucoScreen Plus™ is an improved histochemical staining kit for the determination of peroxidase-positive white blood cells in semen. The kit provides useful information in semen samples where the concentration of round cells exceeds 1 million/ml. LeucoScreen Plus has an equal capability to distinguish between peroxidase-positive and -negative round cells as the LeucoScreen kit (same assay performance), but has the advantage that the substrate is not carcinogenic. The color of the substrate in peroxidase-positive white blood cells is bluish-grey to black instead of brown (LeucoScreen). For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen Plus Reagent 1
Fertipro NV
LeucoScreen Plus™ is an improved histochemical staining kit for the determination of peroxidase-positive white blood cells in semen. The kit provides useful information in semen samples where the concentration of round cells exceeds 1 million/ml. LeucoScreen Plus has an equal capability to distinguish between peroxidase-positive and -negative round cells as the LeucoScreen kit (same assay performance), but has the advantage that the substrate is not carcinogenic. The color of the substrate in peroxidase-positive white blood cells is bluish-grey to black instead of brown (LeucoScreen). For more information, visit the FertiPro website: www.fertipro.com
0
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LeucoScreen Plus
Fertipro NV
LeucoScreen Plus™ is an improved histochemical staining kit for the determination of peroxidase-positive white blood cells in semen. The kit provides useful information in semen samples where the concentration of round cells exceeds 1 million/ml. LeucoScreen Plus has an equal capability to distinguish between peroxidase-positive and -negative round cells as the LeucoScreen kit (same assay performance), but has the advantage that the substrate is not carcinogenic. The color of the substrate in peroxidase-positive white blood cells is bluish-grey to black instead of brown (LeucoScreen).For more information, visit the FertiPro website: www.fertipro.com
0
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VitalScreen Reagent 2
Fertipro NV
VitalScreen is an in vitro diagnostic kit for the semi-quantitative determination of human sperm vitality by dye exclusion.The kit consist of 20ml of 1% Eosin Y in saline (product code: VITAL_1 and 30ml of 5% Nigrosin in saline (product code: VITAL_2).The eosin-nigrosin staining technique is based on the principle that dead cells will take up the eosin and as a result stain red. The nigrosin provides a dark background which makes it easier to assess the slides. The VitalScreen™ is based on the test procedure described in the WHO laboratory manual (2010).VitalScreen™ has a 24 month shelf life from date of produce. Reference is made to the product page of VitalScreen on the FertiPro website: www.fertipro.com
0
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VitalScreen Reagent 1
Fertipro NV
VitalScreen is an in vitro diagnostic kit for the semi-quantitative determination of human sperm vitality by dye exclusion.The kit consist of 20ml of 1% Eosin Y in saline (product code: VITAL_1 and 30ml of 5% Nigrosin in saline (product code: VITAL_2).The eosin-nigrosin staining technique is based on the principle that dead cells will take up the eosin and as a result stain red. The nigrosin provides a dark background which makes it easier to assess the slides. The VitalScreen™ is based on the test procedure described in the WHO laboratory manual (2010).VitalScreen™ has a 24 month shelf life from date of produce. Reference is made to the product page of VitalScreen on the FertiPro website: www.fertipro.com
0
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Spermac Stain - Fix (250ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Stain C (250ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Stain B (250ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Stain A (250ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Fix (50ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Stain C (50ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Stain B (50ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - Stain A (50ml)
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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Spermac Stain - 4x 50ml
Fertipro NV
Spermac Stain is a qualitative diagnostic kit for staining human spermatozoa. The purpose of staining spermatozoa is to be able to differentiate morphologically normal from abnormal sperm cells.For more information, visit the FertiPro website: www.fertipro.com
0
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VitalScreen
Fertipro NV
VitalScreen is an in vitro diagnostic kit for the semi-quantitative determination of human sperm vitality by dye exclusion.The kit consist of 20ml of 1% Eosin Y in saline (product code: VITAL_1 and 30ml of 5% Nigrosin in saline (product code: VITAL_2).The eosin-nigrosin staining technique is based on the principle that dead cells will take up the eosin and as a result stain red. The nigrosin provides a dark background which makes it easier to assess the slides. The VitalScreen™ is based on the test procedure described in the WHO laboratory manual (2010).VitalScreen™ has a 24 month shelf life from date of produce. Reference is made to the product page of VitalScreen on the FertiPro website: www.fertipro.com
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Clarifier™ 2 is an aqueous glacial acetic acid rinse designed to eliminate background staining sometimes caused by excessive adhesives in the water bath such as gelatin. Clarifier™ 2 selectively removes hematoxylin staining from excess adhesive without affecting nuclear staining. This product is only effective with progressive staining an should not be used with regressive stains. Clarifier 2 was designed to be used in histology only. Clarifier™ 2 is designed for use after staining with progressive hematoxylins. After tissues have been sufficiently stained with a progressive hematoxylin, slides are removed from the stain and excess hematoxylin is removed by rinsing the sections in water. The sections are then placed into Clarifier™ 2 for 20 seconds to 40 seconds. A 1-minute water rinse should follow as this stops the reaction and rinses excess acid from the tissue section.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Bluing Reagent is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Bluing Reagent is a buffered alkaline rinse that shifts the final hue of hematoxylin from reddish-blue to traditional blue-purple. Unlike ammonia water and lithium carbonate, Bluing Reagent inhibits pH changes that can adversely affect nuclear detail and “crispness”.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Nu-Clear™ is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Nu-Clear™ is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Bluing Reagent is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Bluing Reagent is a buffered alkaline rinse that shifts the final hue of hematoxylin from reddish-blue to traditional blue-purple. Unlike ammonia water and lithium carbonate, Bluing Reagent inhibits pH changes that can adversely affect nuclear detail and “crispness”.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Bluing Reagent is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Bluing Reagent is a buffered alkaline rinse that shifts the final hue of hematoxylin from reddish-blue to traditional blue-purple. Unlike ammonia water and lithium carbonate, Bluing Reagent inhibits pH changes that can adversely affect nuclear detail and “crispness”.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.
0
-
Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.
0
-
Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.
0
-
Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Modified Grocott’s Methenamine Silver Stain Kit (Chromic Acid) is intended for use as an aid to identify fungal organisms and basement membranes in tissue sections for the diagnosis of general pathology specimens. Fungal cell walls are rich in polysaccharides known as glucans. Treatment with either periodic acid or chromic acid oxidizes these polysaccharides to form aldehyde groups; however, chromic acid is the stronger oxidizer. Chromic acid will achieve a greater degree of oxidation, rendering some chemical groups non-reactive with silver so that they are not demonstrated within the final stain. This greater level of oxidation also results in reduced background staining of basement membranes and collagen fibers, as well as more effective demonstration of Pneumocystis jiroveci (formerly classified as Pneumocystis carinii) and Histoplasma capsulatum (the causative organism of Histoplasmosis). After oxidation, Sodium Metabisulfite Solution removes residual Chromic Acid Solution from the tissue. The aldehyde groups react with the silver ions present in the Working Methenamine Silver Solution and reduce them to form visible metallic silver. Gold Chloride Solution tones the sections and intensifies the reduced silver by forming a silver-gold complex. Sodium Thiosulfate Solution removes any unreduced silver from the tissue sections, followed by Fast Green Stain Solution which produces a light green background to further enhance contrast.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Modified Grocott’s Methenamine Silver Stain Kit (Chromic Acid) is intended for use as an aid to identify fungal organisms and basement membranes in tissue sections for the diagnosis of general pathology specimens. Fungal cell walls are rich in polysaccharides known as glucans. Treatment with either periodic acid or chromic acid oxidizes these polysaccharides to form aldehyde groups; however, chromic acid is the stronger oxidizer. Chromic acid will achieve a greater degree of oxidation, rendering some chemical groups non-reactive with silver so that they are not demonstrated within the final stain. This greater level of oxidation also results in reduced background staining of basement membranes and collagen fibers, as well as more effective demonstration of Pneumocystis jiroveci (formerly classified as Pneumocystis carinii) and Histoplasma capsulatum (the causative organism of Histoplasmosis). After oxidation, Sodium Metabisulfite Solution removes residual Chromic Acid Solution from the tissue. The aldehyde groups react with the silver ions present in the Working Methenamine Silver Solution and reduce them to form visible metallic silver. Gold Chloride Solution tones the sections and intensifies the reduced silver by forming a silver-gold complex. Sodium Thiosulfate Solution removes any unreduced silver from the tissue sections, followed by Fast Green Stain Solution which produces a light green background to further enhance contrast.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Modified Steiner-Steiner Silver Stain Kit aids in identifying spirochetes and non-filamentous bacteria in tissue sections for the diagnosis of general pathology specimens. Microorganisms that stain positively with this technique have a cell wall that will adsorb silver from a silver solution (argyrophilic). The silver is first adsorbed in a non-visible form and is subsequently reduced by the Working Reducing Solution (hydroquinone) to a visible metallic silver.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Elastic Stain Kit and its components are used as a special stain kit to identify elastic fibers. The elastic fibers stain black due to the dye lake created by the hematoxylin, ferric chloride and iodine mixture. The fibers are overstained and then differentiated with ferric chloride to break the dye-lake complex. To ensure fibers are not over-differentiated, slides should be examined during this step under the microscope. Elastic fibers will be stained black and sharply defined. The Van Gieson solution then counterstains the tissue with collagen staining red and other tissue structures, yellow. The Van Gieson solution will also remove some of the elastic fiber staining so the knowledge of a professional is required for adequate use. After staining, care should be taken in choosing a compatible mounting medium as some mountants can leach the picric acid from the Van Gieson counterstain.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Gram Stain Kits – Tissue & Film Bacteria can be classified into one of two families based upon structural and compositional differences in their cell walls. Gram-positive bacteria have thick cell walls with high peptidoglycan content, whereas Gram-negative bacteria have thin cell walls with low peptidoglycan content. Both Gram-positive and Gram-negative bacteria stain with the dye- lake formed by Crystal Violet Solution and Gram’s Iodine Solution. However, during rinsing with Decolorizing Solution, this dye-lake is completely removed from the thin-walled Gram- negative bacteria, allowing them to be subsequently stained with Safranin O Stain Solution. The short duration of the decolorization step enables Gram-positive bacteria to retain the crystal violet dye-lake. Care should be taken when rinsing slides with Decolorizing Solution, as extended rinses can cause the crystal violet dye-lake to be removed from Gram-positive bacteria in addition to Gram-negative bacteria. Within the Tissue Kit only, tissue elements are then counterstained yellow by Tartrazine Stain Solution.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Modified Grocott’s Methenamine Silver Stain Kit (Chromic Acid) is intended for use as an aid to identify fungal organisms and basement membranes in tissue sections for the diagnosis of general pathology specimens. Fungal cell walls are rich in polysaccharides known as glucans. Treatment with either periodic acid or chromic acid oxidizes these polysaccharides to form aldehyde groups; however, chromic acid is the stronger oxidizer. Chromic acid will achieve a greater degree of oxidation, rendering some chemical groups non-reactive with silver so that they are not demonstrated within the final stain. This greater level of oxidation also results in reduced background staining of basement membranes and collagen fibers, as well as more effective demonstration of Pneumocystis jiroveci (formerly classified as Pneumocystis carinii) and Histoplasma capsulatum (the causative organism of Histoplasmosis). After oxidation, Sodium Metabisulfite Solution removes residual Chromic Acid Solution from the tissue. The aldehyde groups react with the silver ions present in the Working Methenamine Silver Solution and reduce them to form visible metallic silver. Gold Chloride Solution tones the sections and intensifies the reduced silver by forming a silver-gold complex. Sodium Thiosulfate Solution removes any unreduced silver from the tissue sections, followed by Fast Green Stain Solution which produces a light green background to further enhance contrast.
0
-
Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Modified Steiner-Steiner Silver Stain Kit aids in identifying spirochetes and non-filamentous bacteria in tissue sections for the diagnosis of general pathology specimens. Microorganisms that stain positively with this technique have a cell wall that will adsorb silver from a silver solution (argyrophilic). The silver is first adsorbed in a non-visible form and is subsequently reduced by the Working Reducing Solution (hydroquinone) to a visible metallic silver.
0
-
Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Modified Steiner-Steiner Silver Stain Kit aids in identifying spirochetes and non-filamentous bacteria in tissue sections for the diagnosis of general pathology specimens. Microorganisms that stain positively with this technique have a cell wall that will adsorb silver from a silver solution (argyrophilic). The silver is first adsorbed in a non-visible form and is subsequently reduced by the Working Reducing Solution (hydroquinone) to a visible metallic silver.
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