BD Cytology Stain Kit

Kit Cytology Stain M

BD Non-GYN Stain Kit

7-AAD Viability Dye

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Tissue Marking Dyes are intended as an aid in the orientation, of excised surgical specimens through frozen sectioning, processing, and sectioning, and subsequent microscopic examination of tissues. The marking of multiple margins with these dyes is especially useful for identification in skin biopsies, a technique known as Moh’s surgery. These dyes are normally applied to fresh tissue but may also be used with fixed tissue. Whether fresh or fixed, the tissue should be blotted to remove excess fluid from the surface before applying the dye. Tissue Marking Dyes will permanently bond to the tissue 1 to 2 minutes after application. The tissue can then be frozen and prepared for sectioning or placed in formalin or other fixatives and processed for paraffin embedding.

Alcian Blue reacts to compounds containing anionic groups such as acid mucosubstances and acid mucins. At pH 2.5, both carboxylated and sulfated acid mucosubstances are stained blue. Periodic acid is used to oxidize the tissue to form aldehyde groups that are demonstrated by Schiff Reagent. Glycogen and neutral mucosubstances as well as basement membranes will be stained magenta by Schiff Reagent, followed by a tap water rinse to develop and intensify its magenta hues. An optional counterstain may be used to provide nuclear detail. Hematoxylin 7211 is recommended; however, Kernechtrot Nuclear Fast Red may be used if a red nuclear stain is desired

Elastic Stain Kit and its components are used as a special stain kit to identify elastic fibers. The elastic fibers stain black due to the dye lake created by the hematoxylin, ferric chloride and iodine mixture. The fibers are overstained and then differentiated with ferric chloride to break the dye-lake complex. To ensure fibers are not over-differentiated, slides should be examined during this step under the microscope. Elastic fibers will be stained black and sharply defined. The Van Gieson solution then counterstains the tissue with collagen staining red and other tissue structures, yellow. The Van Gieson solution will also remove some of the elastic fiber staining so the knowledge of a professional is required for adequate use. After staining, care should be taken in choosing a compatible mounting medium as some mountants can leach the picric acid from the Van Gieson counterstain.

Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.

Elastic Stain Kit and its components are used as a special stain kit to identify elastic fibers. The elastic fibers stain black due to the dye lake created by the hematoxylin, ferric chloride and iodine mixture. The fibers are overstained and then differentiated with ferric chloride to break the dye-lake complex. To ensure fibers are not over-differentiated, slides should be examined during this step under the microscope. Elastic fibers will be stained black and sharply defined. The Van Gieson solution then counterstains the tissue with collagen staining red and other tissue structures, yellow. The Van Gieson solution will also remove some of the elastic fiber staining so the knowledge of a professional is required for adequate use. After staining, care should be taken in choosing a compatible mounting medium as some mountants can leach the picric acid from the Van Gieson counterstain.

The Acid Fast Bacillus Stain Kit (87016) and components are for use as a kit in special stain techniques to detect acid fast bacteria. A waxy, lipid-rich cell wall surrounds the acid fast bacterium. This cell wall absorbs carbol fuchsin but is resistant to decoloration with acid alcohol. Bacteria that do not have a lipid-rich cell wall easily decolorize leaving the acid fast bacillus. Loeffler Methylene Blue Stain Solution provides a light blue counterstain to provide contrast for surrounding tissue elements from the brick red acid fast bacillus.

Elastic Stain Kit and its components are used as a special stain kit to identify elastic fibers. The elastic fibers stain black due to the dye lake created by the hematoxylin, ferric chloride and iodine mixture. The fibers are overstained and then differentiated with ferric chloride to break the dye-lake complex. To ensure fibers are not over-differentiated, slides should be examined during this step under the microscope. Elastic fibers will be stained black and sharply defined. The Van Gieson solution then counterstains the tissue with collagen staining red and other tissue structures, yellow. The Van Gieson solution will also remove some of the elastic fiber staining so the knowledge of a professional is required for adequate use. After staining, care should be taken in choosing a compatible mounting medium as some mountants can leach the picric acid from the Van Gieson counterstain.

Elastic Stain Kit and its components are used as a special stain kit to identify elastic fibers. The elastic fibers stain black due to the dye lake created by the hematoxylin, ferric chloride and iodine mixture. The fibers are overstained and then differentiated with ferric chloride to break the dye-lake complex. To ensure fibers are not over-differentiated, slides should be examined during this step under the microscope. Elastic fibers will be stained black and sharply defined. The Van Gieson solution then counterstains the tissue with collagen staining red and other tissue structures, yellow. The Van Gieson solution will also remove some of the elastic fiber staining so the knowledge of a professional is required for adequate use. After staining, care should be taken in choosing a compatible mounting medium as some mountants can leach the picric acid from the Van Gieson counterstain.

Elastic Stain Kit and its components are used as a special stain kit to identify elastic fibers. The elastic fibers stain black due to the dye lake created by the hematoxylin, ferric chloride and iodine mixture. The fibers are overstained and then differentiated with ferric chloride to break the dye-lake complex. To ensure fibers are not over-differentiated, slides should be examined during this step under the microscope. Elastic fibers will be stained black and sharply defined. The Van Gieson solution then counterstains the tissue with collagen staining red and other tissue structures, yellow. The Van Gieson solution will also remove some of the elastic fiber staining so the knowledge of a professional is required for adequate use. After staining, care should be taken in choosing a compatible mounting medium as some mountants can leach the picric acid from the Van Gieson counterstain.

Gram Stain Kits – Tissue & Film Bacteria can be classified into one of two families based upon structural and compositional differences in their cell walls. Gram-positive bacteria have thick cell walls with high peptidoglycan content, whereas Gram-negative bacteria have thin cell walls with low peptidoglycan content. Both Gram-positive and Gram-negative bacteria stain with the dye- lake formed by Crystal Violet Solution and Gram’s Iodine Solution. However, during rinsing with Decolorizing Solution, this dye-lake is completely removed from the thin-walled Gram- negative bacteria, allowing them to be subsequently stained with Safranin O Stain Solution. The short duration of the decolorization step enables Gram-positive bacteria to retain the crystal violet dye-lake. Care should be taken when rinsing slides with Decolorizing Solution, as extended rinses can cause the crystal violet dye-lake to be removed from Gram-positive bacteria in addition to Gram-negative bacteria. Within the Tissue Kit only, tissue elements are then counterstained yellow by Tartrazine Stain Solution.

Modified Steiner-Steiner Silver Stain Kit aids in identifying spirochetes and non-filamentous bacteria in tissue sections for the diagnosis of general pathology specimens. Microorganisms that stain positively with this technique have a cell wall that will adsorb silver from a silver solution (argyrophilic). The silver is first adsorbed in a non-visible form and is subsequently reduced by the Working Reducing Solution (hydroquinone) to a visible metallic silver.

Modified Steiner-Steiner Silver Stain Kit aids in identifying spirochetes and non-filamentous bacteria in tissue sections for the diagnosis of general pathology specimens. Microorganisms that stain positively with this technique have a cell wall that will adsorb silver from a silver solution (argyrophilic). The silver is first adsorbed in a non-visible form and is subsequently reduced by the Working Reducing Solution (hydroquinone) to a visible metallic silver.

Alcian Blue reacts to compounds containing anionic groups such as acid mucosubstances and acid mucins. At pH 2.5, both carboxylated and sulfated acid mucosubstances are stained blue. Periodic acid is used to oxidize the tissue to form aldehyde groups that are demonstrated by Schiff Reagent. Glycogen and neutral mucosubstances as well as basement membranes will be stained magenta by Schiff Reagent, followed by a tap water rinse to develop and intensify its magenta hues. An optional counterstain may be used to provide nuclear detail. Hematoxylin 7211 is recommended; however, Kernechtrot Nuclear Fast Red may be used if a red nuclear stain is desired

Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.

Potassium Permanganate Solution is the oxidizing agent in this technique. It is followed by an Oxalic Acid Solution to remove the excess permanganate. This step serves to enhance the subsequent staining of the reticular fibers. A sensitizing step using Ferric Ammonium Sulfate Solution impregnates the fibers, creating a metal-organic bond that is replaced by silver during subsequent treatment with Working Ammoniacal Silver Solution. To develop the visible silver, a Reducing Solution of dilute formaldehyde is used. The tissue structures that have been impregnated with the silver solution will now appear brown to black. Gold Chloride Solution is used to “tone” the sections, producing better contrast and clarity as the gold reacts and combines with the reduced silver. Unreduced silver is removed via treatment with Sodium Thiosulfate Solution. An optional nuclear fast red counterstain provides background staining as well as nuclear detail.

The Rapid-Chrome™ H&E Frozen Section Staining Kit simplifies the routine staining of frozen tissue sections from a cryostat. With the Rapid-Chrome™ Staining Kit, all of the reagents needed for H&E-stained frozen sections are supplied in one convenient, portable tray. Reagents are sequentially aligned so that the user can dip slides progressively from jar to jar.