Clear-Rite, Citrus Clearing Solvent, Xylene Substitute, Xylene, and Toluene are a group of solvents known to act as clearing reagents in the histology laboratory for tissue processing and staining. These clearing reagents are used to “clear” tissue or make it permeable to paraffin in tissue processing. In staining, Clearants are used after alcohol which dehydrates the tissue to enable the slide to be cover slipped with a resinous mounting media.

Clear-Rite, Citrus Clearing Solvent, Xylene Substitute, Xylene, and Toluene are a group of solvents known to act as clearing reagents in the histology laboratory for tissue processing and staining. These clearing reagents are used to “clear” tissue or make it permeable to paraffin in tissue processing. In staining, Clearants are used after alcohol which dehydrates the tissue to enable the slide to be cover slipped with a resinous mounting media.

Clear-Rite, Citrus Clearing Solvent, Xylene Substitute, Xylene, and Toluene are a group of solvents known to act as clearing reagents in the histology laboratory for tissue processing and staining. These clearing reagents are used to “clear” tissue or make it permeable to paraffin in tissue processing. In staining, Clearants are used after alcohol which dehydrates the tissue to enable the slide to be cover slipped with a resinous mounting media.

Eosin-Y is an acidified, alcoholic counterstain that provides excellent delineation of cytoplasmic components and stains the cytoplasm of muscle, red blood cells, and connective tissue three distinct color hues of pink to red. Eosin-Y provides excellent contrast between cytoplasmic components and the bluish-purple hues of the nuclear chromatin.

Eosin-Y Alcoholic is a ready-to-use alcoholic, acidified counterstain. This product stains the cytoplasm of muscle, red blood cells, and connective tissue three distinct color hues of pink to red. Eosin-Y Alcoholic provides clear cytoplasmic and nuclear contrast.

Eosin-Y Alcoholic is a ready-to-use alcoholic, acidified counterstain. This product stains the cytoplasm of muscle, red blood cells, and connective tissue three distinct color hues of pink to red. Eosin-Y Alcoholic provides clear cytoplasmic and nuclear contrast.

Eosin-Y Alcoholic is a ready-to-use alcoholic, acidified counterstain. This product stains the cytoplasm of muscle, red blood cells, and connective tissue three distinct color hues of pink to red. Eosin-Y Alcoholic provides clear cytoplasmic and nuclear contrast.

Eosin-Y w/ Phloxine is a ready-to-use alcoholic, acidified counterstain to which phloxine B dye has been added. The addition of phloxine B results in a more intense red coloration of connective tissue, especially muscle, as well as better demonstration of inclusion bodies.

Eosin-Y w/ Phloxine is a ready-to-use alcoholic, acidified counterstain to which phloxine B dye has been added. The addition of phloxine B results in a more intense red coloration of connective tissue, especially muscle, as well as better demonstration of inclusion bodies.

Eosin-Y Saturated is an alcohol-based, rapid cytoplasmic stain. It does not contain glacial acetic acid, so its pH is higher (i.e. more neutral) than other alcoholic eosin Y counterstains. This stain will provide intensities similar to other commercially available eosin Y products.

Hematoxylin 7211 is a rapid, progressive hematoxylin. The product is a hybrid between classical Harris and Gill formulations: it provides the classic intensity and hues of a Harris hematoxylin and the rapid progressive qualities of a Gill hematoxylin. Differentiation is not required, but if desired Clarifier 1 should be used. Clarifier 1 will eliminate any background staining that may be caused by the use of excess gelatin or other adhesives in the water bath. Clarifier 2 can be used as well; however, a shorter immersion time is required. Hematoxylin 7211 does not have an affinity for acid mucopolysaccharide (mucin) staining, as do some other hematoxylin formulations. It provides consistent, well-delineated nuclear staining results.

Hematoxylin 1 is a rapid, progressive hematoxylin designed to produce staining characteristics, intensities, and hues similar to Gill 1 & 2 hematoxylins. Hematoxylin 2 is a darker and more color intensive version of the Hematoxylin 1 stain.

Hematoxylin 1 is a rapid, progressive hematoxylin designed to produce staining characteristics, intensities, and hues similar to Gill 1 & 2 hematoxylins. Hematoxylin 2 is a darker and more color intensive version of the Hematoxylin 1 stain.

Gill Hematoxylin 1, 2, and 3 are rapid, progressive hematoxylins that are widely known and respected in the histology and cytology fields. They offer convenience and choice of stain intensity. Gill hematoxylins are ready to use and will provide familiar, consistent, well-delineated results. Stain filtering may be needed to remove sheen caused by oxidation or cells shed off of slides in the cytology staining process. Gill 1 is the least intense stain and is most commonly used in cytology and in special stains. Gill 2 is a moderately intense stain and is most commonly used in histology, with some limited use in cytology. Gill 3 is an intense stain and is exclusively used in histology as well as staining of plastic sections.

Gill Hematoxylin 1, 2, and 3 are rapid, progressive hematoxylins that are widely known and respected in the histology and cytology fields. They offer convenience and choice of stain intensity. Gill hematoxylins are ready to use and will provide familiar, consistent, well-delineated results. Stain filtering may be needed to remove sheen caused by oxidation or cells shed off of slides in the cytology staining process. Gill 1 is the least intense stain and is most commonly used in cytology and in special stains. Gill 2 is a moderately intense stain and is most commonly used in histology, with some limited use in cytology. Gill 3 is an intense stain and is exclusively used in histology as well as staining of plastic sections.

Gill Hematoxylin 1, 2, and 3 are rapid, progressive hematoxylins that are widely known and respected in the histology and cytology fields. They offer convenience and choice of stain intensity. Gill hematoxylins are ready to use and will provide familiar, consistent, well-delineated results. Stain filtering may be needed to remove sheen caused by oxidation or cells shed off of slides in the cytology staining process. Gill 1 is the least intense stain and is most commonly used in cytology and in special stains. Gill 2 is a moderately intense stain and is most commonly used in histology, with some limited use in cytology. Gill 3 is an intense stain and is exclusively used in histology as well as staining of plastic sections.

Gill Hematoxylin 1, 2, and 3 are rapid, progressive hematoxylins that are widely known and respected in the histology and cytology fields. They offer convenience and choice of stain intensity. Gill hematoxylins are ready to use and will provide familiar, consistent, well-delineated results. Stain filtering may be needed to remove sheen caused by oxidation or cells shed off of slides in the cytology staining process. Gill 1 is the least intense stain and is most commonly used in cytology and in special stains. Gill 2 is a moderately intense stain and is most commonly used in histology, with some limited use in cytology. Gill 3 is an intense stain and is exclusively used in histology as well as staining of plastic sections.

Gill Hematoxylin 1, 2, and 3 are rapid, progressive hematoxylins that are widely known and respected in the histology and cytology fields. They offer convenience and choice of stain intensity. Gill hematoxylins are ready to use and will provide familiar, consistent, well-delineated results. Stain filtering may be needed to remove sheen caused by oxidation or cells shed off of slides in the cytology staining process. Gill 1 is the least intense stain and is most commonly used in cytology and in special stains. Gill 2 is a moderately intense stain and is most commonly used in histology, with some limited use in cytology. Gill 3 is an intense stain and is exclusively used in histology as well as staining of plastic sections.

Gill Hematoxylin 1, 2, and 3 are rapid, progressive hematoxylins that are widely known and respected in the histology and cytology fields. They offer convenience and choice of stain intensity. Gill hematoxylins are ready to use and will provide familiar, consistent, well-delineated results. Stain filtering may be needed to remove sheen caused by oxidation or cells shed off of slides in the cytology staining process. Gill 1 is the least intense stain and is most commonly used in cytology and in special stains. Gill 2 is a moderately intense stain and is most commonly used in histology, with some limited use in cytology. Gill 3 is an intense stain and is exclusively used in histology as well as staining of plastic sections.

Modified Harris Hematoxylin is an acidified, ready-to-use formulation that provides traditional nuclear staining results. Harris hematoxylin procedures require acid alcohol differentiation. The nuclear chromatin and cytoplasm are overstained and the acid alcohol is used to destain the cytoplasm and to remove excess stain from the nucleus. Staining times are typically in the 5-minute range. Epredia Differentiating Solution is recommended for use with Modified Harris Hematoxylin

Modified Harris Hematoxylin is an acidified, ready-to-use formulation that provides traditional nuclear staining results. Harris hematoxylin procedures require acid alcohol differentiation. The nuclear chromatin and cytoplasm are overstained and the acid alcohol is used to destain the cytoplasm and to remove excess stain from the nucleus. Staining times are typically in the 5-minute range. Epredia Differentiating Solution is recommended for use with Modified Harris Hematoxylin

Modified Harris Hematoxylin is an acidified, ready-to-use formulation that provides traditional nuclear staining results. Harris hematoxylin procedures require acid alcohol differentiation. The nuclear chromatin and cytoplasm are overstained and the acid alcohol is used to destain the cytoplasm and to remove excess stain from the nucleus. Staining times are typically in the 5-minute range. Epredia Differentiating Solution is recommended for use with Modified Harris Hematoxylin

Bluing Reagent is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Bluing Reagent is a buffered alkaline rinse that shifts the final hue of hematoxylin from reddish-blue to traditional blue-purple. Unlike ammonia water and lithium carbonate, Bluing Reagent inhibits pH changes that can adversely affect nuclear detail and “crispness”.

Bluing Reagent is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Bluing Reagent is a buffered alkaline rinse that shifts the final hue of hematoxylin from reddish-blue to traditional blue-purple. Unlike ammonia water and lithium carbonate, Bluing Reagent inhibits pH changes that can adversely affect nuclear detail and “crispness”.

Clarifier™ 1 is a reagent designed to eliminate background staining sometimes caused by excessive adhesives in the water bath such as gelatin. Clarifier™ 1 selectively removes hematoxylin staining from excess adhesive without affecting nuclear staining. This product is only effective with progressive staining and should not be used with regressive stains. Clarifier™ 1 is designed for use after staining with progressive hematoxylins. After tissues have been sufficiently stained with a progressive hematoxylin, slides are removed from the stain and excess hematoxylin is removed by rinsing the sections in water. The sections are then placed into Clarifier™ 1 for 30 seconds to 1 minute. A 1-minute water rinse should follow as this stops the reaction and rinses excess acid from the tissue section. If Clarifier™ 1 is not successful in removing the background staining, use Clarifier™ 2 in its place with a possible time reduction.

Clarifier™ 2 is an aqueous glacial acetic acid rinse designed to eliminate background staining sometimes caused by excessive adhesives in the water bath such as gelatin. Clarifier™ 2 selectively removes hematoxylin staining from excess adhesive without affecting nuclear staining. This product is only effective with progressive staining an should not be used with regressive stains. Clarifier 2 was designed to be used in histology only. Clarifier™ 2 is designed for use after staining with progressive hematoxylins. After tissues have been sufficiently stained with a progressive hematoxylin, slides are removed from the stain and excess hematoxylin is removed by rinsing the sections in water. The sections are then placed into Clarifier™ 2 for 20 seconds to 40 seconds. A 1-minute water rinse should follow as this stops the reaction and rinses excess acid from the tissue section.

Differentiating Solution is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Differentiating Solution is an acid alcohol solution to be used with the Modified Harris Hematoxylin. It is formulated as a standard 1% hydrochloric acid in 70% alcohol solution and will provide consistent differentiation results. Differentiating Solution’s mode of action is that it aggressively reacts with the tissue dye-lake complex in nuclear chromatin, resulting in the removal of the stain. Due to weak bonding in the cytoplasm, hematoxylin in this area is also easily and completely removed. Generally, two to four quick dips will be adequate for differentiation when using Differentiating Solution, followed by a 1-minute water rinse to stop the reaction and fully remove the acid from the tissue.

Differentiating Solution is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Differentiating Solution is an acid alcohol solution to be used with the Modified Harris Hematoxylin. It is formulated as a standard 1% hydrochloric acid in 70% alcohol solution and will provide consistent differentiation results. Differentiating Solution’s mode of action is that it aggressively reacts with the tissue dye-lake complex in nuclear chromatin, resulting in the removal of the stain. Due to weak bonding in the cytoplasm, hematoxylin in this area is also easily and completely removed. Generally, two to four quick dips will be adequate for differentiation when using Differentiating Solution, followed by a 1-minute water rinse to stop the reaction and fully remove the acid from the tissue.

Clarifier™ 1 is a reagent designed to eliminate background staining sometimes caused by excessive adhesives in the water bath such as gelatin. Clarifier™ 1 selectively removes hematoxylin staining from excess adhesive without affecting nuclear staining. This product is only effective with progressive staining and should not be used with regressive stains. Clarifier™ 1 is designed for use after staining with progressive hematoxylins. After tissues have been sufficiently stained with a progressive hematoxylin, slides are removed from the stain and excess hematoxylin is removed by rinsing the sections in water. The sections are then placed into Clarifier™ 1 for 30 seconds to 1 minute. A 1-minute water rinse should follow as this stops the reaction and rinses excess acid from the tissue section. If Clarifier™ 1 is not successful in removing the background staining, use Clarifier™ 2 in its place with a possible time reduction.

Clarifier™ 2 is an aqueous glacial acetic acid rinse designed to eliminate background staining sometimes caused by excessive adhesives in the water bath such as gelatin. Clarifier™ 2 selectively removes hematoxylin staining from excess adhesive without affecting nuclear staining. This product is only effective with progressive staining an should not be used with regressive stains. Clarifier 2 was designed to be used in histology only. Clarifier™ 2 is designed for use after staining with progressive hematoxylins. After tissues have been sufficiently stained with a progressive hematoxylin, slides are removed from the stain and excess hematoxylin is removed by rinsing the sections in water. The sections are then placed into Clarifier™ 2 for 20 seconds to 40 seconds. A 1-minute water rinse should follow as this stops the reaction and rinses excess acid from the tissue section.

Cyto-Stain™ G is a modification of Cyto-Stain™, designed to produce greener cyanophilic hues in intermediate and basal cells, and is used in the same manner as Cyto-Stain™.

Cyto-Stain™ acts as a single-solution cytoplasmic stain, producing the full range of familiar colors seen in conventional Papanicolaou staining and providing complete control of staining intensity. The staining of Cyto-Stain™ is accomplished through a formulation based upon the differential affinities of its component dyes for various cellular components. Final cytoplasmic hues are influenced by both the prior action of Clarifier 1 and the subsequent 95% alcohol rinses. Cyto-Stain™ produces results comparable to Pap staining with one minor difference: cilia in bronchial washings stain blue-green with Cyto-Stain™ rather than red as seen in traditional Pap staining procedures

Cyto-Stain™ acts as a single-solution cytoplasmic stain, producing the full range of familiar colors seen in conventional Papanicolaou staining and providing complete control of staining intensity. The staining of Cyto-Stain™ is accomplished through a formulation based upon the differential affinities of its component dyes for various cellular components. Final cytoplasmic hues are influenced by both the prior action of Clarifier 1 and the subsequent 95% alcohol rinses. Cyto-Stain™ produces results comparable to Pap staining with one minor difference: cilia in bronchial washings stain blue-green with Cyto-Stain™ rather than red as seen in traditional Pap staining procedures

OG-6 and Modified OG are ready-to-use cytoplasmic counterstains that stain keratinized cells orange. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results.OG-6 has a specific affinity for keratinized cells and will not stain other cellular constituents. When used in conjunction with EA stains, the end user can expect traditional staining results that are consistent and familiar. Stain filtering is recommended to remove cells shed off of slides in the cytology staining process.

OG-6 is a traditional formulation that, when used with various EA products, will provide familiar staining results for gynecological and non-gynecological specimens.

OG-6 is a traditional formulation that, when used with various EA products, will provide familiar staining results for gynecological and non-gynecological specimens.

Modified OG is used in conjunction with EA products for staining gynecological and non-gynecological specimens. This formulation is optimized to reduce precipitate formation and staining times common with traditional OG-6 formulations. Modified OG and Modified EA, when used together, will reduce filtering and staining times.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA-36 is used in conjunction with OG-6 for the staining of gynecological specimens. It will provide a slightly lighter cytoplasmic hue than EA-50. EA-36 is recommended for end users who desire a more pastel staining result in gynecological specimens.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA-36 is used in conjunction with OG-6 for the staining of gynecological specimens. It will provide a slightly lighter cytoplasmic hue than EA-50. EA-36 is recommended for end users who desire a more pastel staining result in gynecological specimens.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA 50 is used in conjunction with OG-6 for the staining of gynecological specimens. It will provide a very traditional staining result with common staining hues and intensities. EA-50 can also be used for non-gynecological specimens however, although EA-65 is recommended.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA 50 is used in conjunction with OG-6 for the staining of gynecological specimens. It will provide a very traditional staining result with common staining hues and intensities. EA-50 can also be used for non-gynecological specimens however, although EA-65 is recommended.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA 50 is used in conjunction with OG-6 for the staining of gynecological specimens. It will provide a very traditional staining result with common staining hues and intensities. EA-50 can also be used for non-gynecological specimens however, although EA-65 is recommended.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA-65 is used in conjunction with OG-6 for the staining of non-gynecological specimens. This formulation contains less dye resulting in lighter staining of highly mucoid sample types common in non-gynecological cytology.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA-65 is used in conjunction with OG-6 for the staining of non-gynecological specimens. This formulation contains less dye resulting in lighter staining of highly mucoid sample types common in non-gynecological cytology.

EA-36, -50, -65 and Modified EA are ready-to-use cytoplasmic counterstains used for gynecological and non-gynecological cytology specimens. These products contain common dyes and reagent solvents used in traditional EA formulations. A well-preserved or fixed slide with adequate exposure to 95% alcohol or a commercial spray fixative is highly recommended for optimal staining results. Cellular results are traditional as well, with basal or intermediate cells staining a blue-green hue and surface cells staining a pink-red hue. This multicolored effect is due to dye uptake by various cells and their affinities for the specific dyes.EA-65 is used in conjunction with OG-6 for the staining of non-gynecological specimens. This formulation contains less dye resulting in lighter staining of highly mucoid sample types common in non-gynecological cytology.

Modified EA is used in conjunction with OG-6 or Modified OG for gynecological and non-gynecological specimens. This formulation does not contain Bismarck Brown and is optimized to reduce precipitate formation that is common with other traditional EA formulations. The Modified EA staining results are most similar to EA-50.

Fixation is the initial and most important step of histology specimen preparation in pathology. 10% Neutral Buffered Formalin is the most used fixative in the histology laboratory, but in certain circumstances, other fixatives may be desired according to tissue type or subsequent pretreatment or staining technique. Zinc Buffered Formalin maintains the traditional formulation of 10% Neutral Buffered Formalin but with the addition of zinc. The addition of zinc provides better nuclear detail in tissue samples.

Fixation is vital in the stabilization and maintenance of the existing form and structure of all cellular constituents. The fixative, when applied, coats, and protects cells with a water-soluble film. This film, derived from resin, coats cell preparations to preserve nuclear detail for microscopic examination and diagnosis.

Fixation is vital in the stabilization and maintenance of the existing form and structure of all cellular constituents. The fixative, when applied, coats, and protects cells with a water-soluble film. This film, derived from resin, coats cell preparations to preserve nuclear detail for microscopic examination and diagnosis.

Fixation is vital in the stabilization and maintenance of the existing form and structure of all cellular constituents. The fixative, when applied, coats, and protects cells with a water-soluble film. This film, derived from resin, coats cell preparations to preserve nuclear detail for microscopic examination and diagnosis.