KOVA STAIN (3 x 25mL)

KOVA Refractrol SP, Low Level

KOVA Refractrol SP, Normal Level

KOVA Refractrol SP, High Level

KOVA Refractrol, High 9.0

KOVA Refractrol, Low 6.0

KOVA STAIN (3X25 ML)

KOVA Stain

For use in the Gram’s Staining method for the initial differentiation of Gram Positive and Gram Negative bacteria.

Gram Stain Kits – Tissue & Film Bacteria can be classified into one of two families based upon structural and compositional differences in their cell walls. Gram-positive bacteria have thick cell walls with high peptidoglycan content, whereas Gram-negative bacteria have thin cell walls with low peptidoglycan content. Both Gram-positive and Gram-negative bacteria stain with the dye- lake formed by Crystal Violet Solution and Gram’s Iodine Solution. However, during rinsing with Decolorizing Solution, this dye-lake is completely removed from the thin-walled Gram- negative bacteria, allowing them to be subsequently stained with Safranin O Stain Solution. The short duration of the decolorization step enables Gram-positive bacteria to retain the crystal violet dye-lake. Care should be taken when rinsing slides with Decolorizing Solution, as extended rinses can cause the crystal violet dye-lake to be removed from Gram-positive bacteria in addition to Gram-negative bacteria. Within the Tissue Kit only, tissue elements are then counterstained yellow by Tartrazine Stain Solution.

Gram Stain Kits – Tissue & Film Bacteria can be classified into one of two families based upon structural and compositional differences in their cell walls. Gram-positive bacteria have thick cell walls with high peptidoglycan content, whereas Gram-negative bacteria have thin cell walls with low peptidoglycan content. Both Gram-positive and Gram-negative bacteria stain with the dye- lake formed by Crystal Violet Solution and Gram’s Iodine Solution. However, during rinsing with Decolorizing Solution, this dye-lake is completely removed from the thin-walled Gram- negative bacteria, allowing them to be subsequently stained with Safranin O Stain Solution. The short duration of the decolorization step enables Gram-positive bacteria to retain the crystal violet dye-lake. Care should be taken when rinsing slides with Decolorizing Solution, as extended rinses can cause the crystal violet dye-lake to be removed from Gram-positive bacteria in addition to Gram-negative bacteria. Within the Tissue Kit only, tissue elements are then counterstained yellow by Tartrazine Stain Solution.

Crystal Violet Working Solution

Crystal Violet, BSC Certified

Crystal Violet Solution, 0.1% Aqueous

Crystal Violet, 2% Aqueous Solution

Crystal Violet Gram's--Crystal Violet 2.5% in 10% Alcohol

Crystal Violet Staining Solution, 1% Aqueous

Hucker's Crystal Violet Solution

Stirling Crystal Violet Solution

Lillie's Crystal Violet Solution

Turk's Crystal Violet Solution