The ALPCO Calprotectin Chemiluminescence ELISA is an in vitro diagnostic chemiluminescent assay intended for the quantitative measurement of fecal calprotectin, a neutrophilic protein that is a marker of intestinal mucosal inflammation, in human stool. The ALPCO Calprotectin Chemiluminescence ELISA is intended for in vitro diagnostic use as an aid in the diagnosis of inflammatory bowel disease (IBD), specifically Crohn’s disease (CD) and ulcerative colitis (UC), and as an aid in the differentiation of IBD from irritable bowel syndrome (IBS) in conjunction with other clinical and laboratory findings.

The ALPCO Calprotectin Chemiluminescence ELISA is an in vitro diagnostic chemiluminescent assay intended for the quantitative measurement of fecal calprotectin, a neutrophilic protein that is a marker of intestinal mucosal inflammation, in human stool. The ALPCO Calprotectin Chemiluminescence ELISA is intended for in vitro diagnostic use as an aid in the diagnosis of inflammatory bowel disease (IBD), specifically Crohn’s disease (CD) and ulcerative colitis (UC), and as an aid in the differentiation of IBD from irritable bowel syndrome (IBS) in conjunction with other clinical and laboratory findings.

Reagent kit for quantitative PETIA assay for the determination of calprotectin (MRP8/14) in human stool specimens

Quantitative ELISA assay for the determination of calprotectin (MRP8/14) in human stool specimens

Fecal extraction device

1. Antibody coated plate: 12-1 x 8 wells.2. Enzyme conjugate antibody (IgG):1x15mL vial.3. Substrate: 1 x15 ml vial.4. Washing solution (20x): 1x50mL vial.5. Dilution solution (10x): 1 x20mL vial.6. Extraction solution (2.5x): 2x50 mL vials.7. Calibrators: 5 vials containing 1.0 ml Calprotectin at five known concentrations (6.25, 12.5, 25, 50, and 100 ng/ml).8. Control 1: 1x1.0 mL vial.9. Control 2: 1x1.0 mL vial.10. Microtiter plate cover: 1 piece.

1. Antibody coated plate: 12-1 x 8 wells.2. Enzyme conjugate antibody (IgG):1x15mL vial.3. Substrate: 1 x15 ml vial.4. Washing solution (20x): 1x50mL vial.5. Dilution solution (10x): 1 x20mL vial.6. Extraction solution (2.5x): 2x50 mL vials.7. Calibrators: 6 vials containing 1.0 ml Calprotectin at six known concentrations (6.25, 12.5, 25, 50, 100 and 200 ng/ml).8. Control 1: 1x1.0 mL vial.9. Control 2: 1x1.0 mL vial.10. Microtiter plate cover: 1 piece.

1. Antibody coated plate: 12-1 x 8 wells.2. Enzyme conjugate antibody (IgG):1x15mL vial.3. Substrate: 1 x15 ml vial.4. Washing solution (20x): 1x50mL vial.5. Dilution solution (10x): 1 x20mL vial.6. Extraction solution (2.5x): 2x50 mL vials.7. Calibrators: 6 vials containing 1.5 ml Calprotectin at six known concentrations (0, 2.5, 12.5, 25, 50 and 150 ng/ml).8. Control 1: 1x1.5 mL vial.9. Control 2: 1x1.5 mL vial.10. Stop Solution: 1x15 mL vial10. Microtiter plate cover: 1 piece.

-100 ea. fecal extraction devices containing 2.8 mL of extraction buffer-100 ea. white caps

1. 1 QUANTA Flash Calprotectin Reagent Cartridge2. 1 QUANTA Flash Special Wash

1. QUANTA Flash Calprotectin Extraction Buffer (2.5X): Two (2) labeled bottles containing 125 mL, concentrated reagent.

1. Antibody coated plate: 12-1 x 8 wells.2. Enzyme conjugate antibody (IgG):1x15mL vial.3. Substrate: 1 x15 ml vial.4. Washing solution (20x): 1x50mL vial.5. Dilution solution (10x): 1 x20mL vial.6. Extraction solution (2.5x): 2x50 mL vials.7. Calibrators: 6 vials containing 1.0 ml Calprotectin at six known concentrations (6.25, 12.5, 25, 50, 100 and 200 ng/ml).8. Control 1: 1x1.0 mL vial.9. Control 2: 1x1.0 mL vial.

1. Antibody coated plate12x8 wells coated with antibody against calprotectin. Plastic sealed bag containing a desiccant.2. Enzyme conjugate antibody (IgG)1 vial containing 15 ml Horse-radish peroxidase-labeled rabbit anti-human calprotectin IgG antibodies in a buffer solution with Proclin 300 as a preservative. Ready-to-use.3. Substrate1 vial containing 15 ml substrate reagent (TMB). Ready-to-use4. Stop Solution1 vial containing 15 ml H2SO4 (0.5M). Ready-to-use5. Washing solution (20x)1 vial containing 50 ml concentrated washing solution. To be diluted with distilled water.6. Diluent solution (10x)1 vial containing 20 ml concentrated diluent solution to be diluted with distilled water.7. Extraction solution (2.5x)2 vials containing 100 ml concentrated extraction solution to be diluted with distilled water. This concentrated solution is irritating to eyes and skin.8. Calibrators6 vials containing 1.5 ml Calprotectin solution at six known concentrations (0, 2.5, 12.5, 25, 50, 150 ng/ml). The value of each Calibrator is printed on the vial label. Ready-to-use.9. Control 11 vial containing 1.5 ml of control 1. Ready-to-use. Do not dilute. The range of values is printed on the vial label.10. Control 21 vial containing 1.5 ml of control 2. Ready-to-use. Do not dilute. The range of values is printed on the vial label.