TRANSENE COMPANY, INC. SO-1068 Rowley Biochemical Chabot Schiff's Reagent

Periodic Acid-Schiff Stain Kit is a staining kit used to detect carbohydrates, glycogen, basement membranes, and fungus in tissue sections for the diagnosis of general pathology specimens. Periodic acid oxidizes these various tissue moieties to aldehydes, to which Schiff Reagent bonds in its colorless form. Rinsing with water removes the sulfurous acid and restores the Schiff Reagent to its rose-colored form.

Alcian Blue reacts to compounds containing anionic groups such as acid mucosubstances and acid mucins. At pH 2.5, both carboxylated and sulfated acid mucosubstances are stained blue. Periodic acid is used to oxidize the tissue to form aldehyde groups that are demonstrated by Schiff Reagent. Glycogen and neutral mucosubstances as well as basement membranes will be stained magenta by Schiff Reagent, followed by a tap water rinse to develop and intensify its magenta hues. An optional counterstain may be used to provide nuclear detail. Hematoxylin 7211 is recommended; however, Kernechtrot Nuclear Fast Red may be used if a red nuclear stain is desired

H&E Stain Kit #2