Hematology
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Hematology
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22,644 Results
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Clarifier™ 2 is an aqueous glacial acetic acid rinse designed to eliminate background staining sometimes caused by excessive adhesives in the water bath such as gelatin. Clarifier™ 2 selectively removes hematoxylin staining from excess adhesive without affecting nuclear staining. This product is only effective with progressive staining an should not be used with regressive stains. Clarifier 2 was designed to be used in histology only. Clarifier™ 2 is designed for use after staining with progressive hematoxylins. After tissues have been sufficiently stained with a progressive hematoxylin, slides are removed from the stain and excess hematoxylin is removed by rinsing the sections in water. The sections are then placed into Clarifier™ 2 for 20 seconds to 40 seconds. A 1-minute water rinse should follow as this stops the reaction and rinses excess acid from the tissue section.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Bluing Reagent is intended to be used as an aid in hematoxylin staining for the diagnosis of general pathology specimens. Bluing Reagent is a buffered alkaline rinse that shifts the final hue of hematoxylin from reddish-blue to traditional blue-purple. Unlike ammonia water and lithium carbonate, Bluing Reagent inhibits pH changes that can adversely affect nuclear detail and “crispness”.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Hematoxylin 1 is a rapid, progressive hematoxylin designed to produce staining characteristics, intensities, and hues similar to Gill 1 & 2 hematoxylins. Hematoxylin 2 is a darker and more color intensive version of the Hematoxylin 1 stain.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Hematoxylin 1 is a rapid, progressive hematoxylin designed to produce staining characteristics, intensities, and hues similar to Gill 1 & 2 hematoxylins. Hematoxylin 2 is a darker and more color intensive version of the Hematoxylin 1 stain.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Hematoxylin 7211 is a rapid, progressive hematoxylin. The product is a hybrid between classical Harris and Gill formulations: it provides the classic intensity and hues of a Harris hematoxylin and the rapid progressive qualities of a Gill hematoxylin. Differentiation is not required, but if desired Clarifier 1 should be used. Clarifier 1 will eliminate any background staining that may be caused by the use of excess gelatin or other adhesives in the water bath. Clarifier 2 can be used as well; however, a shorter immersion time is required. Hematoxylin 7211 does not have an affinity for acid mucopolysaccharide (mucin) staining, as do some other hematoxylin formulations. It provides consistent, well-delineated nuclear staining results.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Eosin-Y is an acidified, alcoholic counterstain that provides excellent delineation of cytoplasmic components and stains the cytoplasm of muscle, red blood cells, and connective tissue three distinct color hues of pink to red. Eosin-Y provides excellent contrast between cytoplasmic components and the bluish-purple hues of the nuclear chromatin.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Hematoxylin 7212 is a darker and more color intensive version of Hematoxylin 7211. It contains most of the attributes and features of the Hematoxylin 7211 stain; however, Hematoxylin 7212 does have a slight affinity for mucin staining.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Gram Stain Kits – Tissue & Film Bacteria can be classified into one of two families based upon structural and compositional differences in their cell walls. Gram-positive bacteria have thick cell walls with high peptidoglycan content, whereas Gram-negative bacteria have thin cell walls with low peptidoglycan content. Both Gram-positive and Gram-negative bacteria stain with the dye- lake formed by Crystal Violet Solution and Gram’s Iodine Solution. However, during rinsing with Decolorizing Solution, this dye-lake is completely removed from the thin-walled Gram- negative bacteria, allowing them to be subsequently stained with Safranin O Stain Solution. The short duration of the decolorization step enables Gram-positive bacteria to retain the crystal violet dye-lake. Care should be taken when rinsing slides with Decolorizing Solution, as extended rinses can cause the crystal violet dye-lake to be removed from Gram-positive bacteria in addition to Gram-negative bacteria. Within the Tissue Kit only, tissue elements are then counterstained yellow by Tartrazine Stain Solution.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Gram Stain Kits – Tissue & Film Bacteria can be classified into one of two families based upon structural and compositional differences in their cell walls. Gram-positive bacteria have thick cell walls with high peptidoglycan content, whereas Gram-negative bacteria have thin cell walls with low peptidoglycan content. Both Gram-positive and Gram-negative bacteria stain with the dye- lake formed by Crystal Violet Solution and Gram’s Iodine Solution. However, during rinsing with Decolorizing Solution, this dye-lake is completely removed from the thin-walled Gram- negative bacteria, allowing them to be subsequently stained with Safranin O Stain Solution. The short duration of the decolorization step enables Gram-positive bacteria to retain the crystal violet dye-lake. Care should be taken when rinsing slides with Decolorizing Solution, as extended rinses can cause the crystal violet dye-lake to be removed from Gram-positive bacteria in addition to Gram-negative bacteria. Within the Tissue Kit only, tissue elements are then counterstained yellow by Tartrazine Stain Solution.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
Hematology stains are typically mixtures of several thiazin dyes in a methanol solvent. Ionic and non-ionic forces are involved in the binding of the dyes. The negatively charged phosphoric acid groups of DNA attract the purple polychromatic cationic dyes to the nuclei. The blue basophilic granules are stained by the polychromatic cationic dyes. Cationic cellular components, such as the erythrocytes and eosinophilic granules, are stained by the red and pink anionic dyes. The buffers used in the staining procedure liberate and activate dye ions allowing them to chemically bond with specific cellular components. When staining blood and bone marrow smears, the pH of the staining solution and/or buffer is a critical factor. The Wright, Wright-Giemsa, Giemsa, May-Grunwald and Jenner stains all rely on a separate buffer solution to control the pH of the reaction, whereas the buffered versions are referred to as “one-step” and have the buffering component already in the staining solution. There are pros and cons to each type of hematology stain although they all contain the thiazin dyes which control the coloration of the nuclear cell components. The single solutions with buffer included generally present more precipitate and the cellular detail is not defined as well as using separate stain and buffer solutions. This kit contains three 500ml bottles of Wright Giemsa stain, pH 6.8 Buffer and a Rinse solution intended to be used as a system per the instructions for use.
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
This staining kit simplifies quick differential staining blood smears and fine needle aspirates with its compact size making it specially ideal for FNA carts. With the Rapid-Chrome Kwik- Diff Staining Kit, all of the necessary reagents and accessories are supplied in one convenient, portable tray. Reagents are sequentially aligned so that the user can dip slides progressively from jar to jar. Additionally, die-cut slots in the tray base accommodate jar lids in order to reduce potential cross-contamination of reagents as well as required counter space. This kit can stain approximately 100 slides, although the actual number may vary based upon sample type and thickness.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
This staining kit simplifies quick differential staining blood smears and fine needle aspirates with its compact size making it specially ideal for FNA carts. With the Rapid-Chrome Kwik- Diff Staining Kit, all of the necessary reagents and accessories are supplied in one convenient, portable tray. Reagents are sequentially aligned so that the user can dip slides progressively from jar to jar. Additionally, die-cut slots in the tray base accommodate jar lids in order to reduce potential cross-contamination of reagents as well as required counter space. This kit can stain approximately 100 slides, although the actual number may vary based upon sample type and thickness.
0
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Epredia
RICHARD-ALLAN SCIENTIFIC COMPANY
This staining kit simplifies quick differential staining blood smears and fine needle aspirates with its compact size making it specially ideal for FNA carts. With the Rapid-Chrome Kwik- Diff Staining Kit, all of the necessary reagents and accessories are supplied in one convenient, portable tray. Reagents are sequentially aligned so that the user can dip slides progressively from jar to jar. Additionally, die-cut slots in the tray base accommodate jar lids in order to reduce potential cross-contamination of reagents as well as required counter space. This kit can stain approximately 100 slides, although the actual number may vary based upon sample type and thickness.
